Close

Loizos Savva

Bioinformatician

Download Resume

About Me

I am a Postdoctoral Researcher in Bioinformatics at the John Innes Centre with a strong background in computational chemistry. My PhD at Cardiff University focused on using molecular dynamics simulations to investigate proteins implicated in neurodegenerative diseases. Now, I apply my computational expertise to plant pathogen genomics, developing bioinformatics pipelines for analyzing next-generation sequencing data. My current work is centered on understanding and combating devastating crop diseases like wheat rust by providing real-time diagnostic solutions and supporting research into pathogen resistance.

Experience

John Innes Centre

Postdoctoral Researcher in Bioinformatics

  • Led development of the Snakemake-based MARPLE diagnostics pipeline for rapid strain-level identification of wheat rust pathogens from field samples using Oxford Nanopore sequencing, deployed in collaboration with partners across East Africa and South Asia.
  • Supported collaborative genomic surveillance efforts in Bhutan, including sequencing and analysis of 13 wheat landraces and contributing to downstream population genomic interpretation with local partners.
  • Worked across Nanopore, RNA-seq, and targeted sequencing datasets, contributing to analyses of pathogen diversity, genome variation, and population structure.
  • Developed a Python-based interface for the MARPLE pipeline, enabling non-specialist users to run analyses and record structured metadata through a barcode-based sample tracking system.
  • Provided bioinformatics support across wheat rust research projects in the Saunders’ Lab, including genomic and epigenetic analyses of pathogen populations and associated host–pathogen interactions.
  • Collaborated with internal and international partners to investigate wheat susceptibility genes involved in resistance to wheat blast.
  • Completed a de novo genome assembly of P. berberata for a conservation-focused research project, integrating sequencing and assembly validation workflows.
  • Designed and delivered bioinformatics training workshops in Linux, R, and NGS analysis, both online and in-person, for researchers and students with diverse backgrounds.

Cardiff University

Graduate Demonstrator

Demonstrated and tutored sessions in Computational, Physical and Inorganic Chemistry and Spectroscopy.

Emphasis Research Centre, University of Cyprus

Research Intern

Project: Detection of nicotine using HS-SPME-GC-MS and SERS.

Education

Cardiff University

Oct 2020 - Nov 2023

PhD in Computational Chemistry

University of Surrey

Sept 2017 - Jun 2020

BSc(Hons) in Chemistry

Publications

A wheat MYB transcription factor activates defense by repressing TaSIZ1 in response to branched-chain amino acid accumulation Published: 2026

Puthanvila-Surendrababu S, Savva L, Korolev A, Hill L, Saalbach G, Saunders DGO

The Plant Cell. DOI: https://doi.org/10.1093/plcell/koag135

Read Publication

Virulence gains in the Puccinia striiformis f. sp. tritici PstS10 lineage correlate with expression polymorphism in a candidate Avr effector Published: 2026

Domingues-Carvalho R, Savva L, Rodriguez-Algaba J, Korolev A, Stephens C, Bryan A, Justesen AF, Hovmøller MS, Saunders DGO

Communications Biology. DOI: https://doi.org/10.1038/s42003-026-10018-0

Read Publication

Genetic analysis of Puccinia graminis f. sp. tritici in Bhutan highlights the need for increased surveillance Published: 2026

Tshomo, Lhamo Y, Wouters R, Hodson DP, Saunders DGO, Savva L, Dema Y

Zenodo. DOI: https://doi.org/10.5281/zenodo.18181952

Read Publication

Genetic analysis of the wheat yellow rust pathogen Puccinia striiformis f. sp. tritici in Bhutan reveals high levels of diversity Published: 2025

Tshomo, Lhamo Y, Wouters R, Hodson DP, Saunders DGO, Savva L, Dema Y

Zenodo. DOI: https://doi.org/10.5281/zenodo.16743539

Read Publication

k-mer-based GWAS reveals a candidate avirulence gene and structural variation in Puccinia triticina linked to gain of Lr20 virulence

Published: 2025

Tsushima A, Morier-Gxoyiya C, Savva L, Czajowski G, Wilderspin S, Czembor P, Holdgate S, Saunders DGO

BMC Genomics. DOI: https://doi.org/10.1186/s12864-025-12230-4

Read Publication

First Large‐Scale Breakdown of Yr15 Resistance to Wheat Yellow Rust (Puccinia striiformis f. sp. tritici) Recorded Globally

Published: 2025

Davis HR, Chia T, Rhodes H, Leaman C, Lewis CM, Savva L, Korolev A, Boyd L, Saunders DGO, Nellist CF, Kanyuka K

New Disease Reports. DOI: https://doi.org/10.1002/ndr2.70067

Read Publication

A portable, nanopore-based genotyping platform for near real-time detection of Puccinia graminis f. sp. tritici lineages and fungicide sensitivity

Published: 2025

Savva L, Bryan A, Vinopal D, Gonzalez-Navarro O, Kosgey Z, Ndungu Cyrus K, Horo Tola J, Danu Gutu K, Molla M, Alemayehu Y, Hodson DP, Saunders DGO

BMC Genomics. DOI: https://doi.org/10.1186/s12864-025-11428-w

Read Publication

A recent shift in the Puccinia striiformis f. sp. tritici population in Serbia coincides with changes in yield losses of commercial winter wheat varieties

Published: 2024

Župunski V, Savva L, Saunders DGO, Jevtić R

Frontiers in Plant Science. DOI: https://doi.org/10.3389/fpls.2024.1464454

Read Publication

Computational investigation of copper-mediated conformational changes in α-synuclein dimer

Published: 2024

Savva L, Platts JA

Physical Chemistry Chemical Physics. DOI: https://doi.org/10.1039/D3CP04697D

Read Publication

Exploring the impact of mutation and post-translational modification on α-Synuclein: Insights from molecular dynamics simulations with and without copper

Published: 2023

Savva L, Platts JA

Journal of Inorganic Biochemistry. DOI: https://doi.org/10.1016/j.jinorgbio.2023.112395

Read Publication

Evaluation of implicit solvent models in molecular dynamics simulation of α-Synuclein

Published: 2023

Savva L, Platts JA

Journal of Biomolecular Structure and Dynamics. DOI: https://doi.org/10.1080/07391102.2022.2082534

Read Publication

How Cu(II) binding affects structure and dynamics of α-synuclein revealed by molecular dynamics simulations

Published: 2023

Savva L, Platts JA

Journal of Inorganic Biochemistry. DOI: https://doi.org/10.1016/j.jinorgbio.2022.112068

Read Publication

Forcefield evaluation and accelerated molecular dynamics simulation of Zn(II) binding to N-terminus of amyloid-β

Published: 2021

Alshammari N, Savva L, Kennedy-Britten O, Platts JA

Computational Biology and Chemistry. DOI: https://doi.org/10.1016/j.compbiolchem.2021.107540

Read Publication

Projects

MARPLE

MARPLE (Mobile And Real-time PLant disEase) is a portable diagnostics pipeline for the rapid, in-field identification of wheat rust pathogens. Packaged using Snakemake, it utilizes data from Nanopore MinION sequencing devices to provide strain-level identification and assess fungicide sensitivity directly from infected samples, facilitating timely disease management in remote agricultural settings.

View Project

Skills

Get in Touch